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Journal

An eco-concerned development of a fast, precise and economical spectrophotometric assay for the antiviral drug simeprevir based on ion-pair formation

By
Mohamed S.H.
Issa Y.M.
Salim A.I.

Simeprevir sodium (SMV) is one of the antiviral drugs used for the treatment of virus C. The current strategy develops and validates a new eco-concerned tool for its quantification in the pure and pharmaceutical formulations. Sulfonephthalein acid dyes were used for this purpose, applying visible analyses based on ion-pair formation. A linear relation between the absorbed signal and the drug concentration is obtained up to 67.0 μg mL-1 with r2 of 0.9989-0.9999. The measurement is carried out at 410, 415, 410, and 403 nm for bromocresol green, bromoxelenol blue, bromothymol blue, and bromocresol purple, respectively, in dichloromethane as a solvent. The drug structure was confirmed utilizing different tools; mass spectrometry, FT-IR, 1H NMR, and thermal analysis. Association, formation constants, molar absorptivity, and free energy change for SMV-ion-pairs were calculated. The limit of detection reaches 50.0 ng mL-1 with a quantification limit of 180.0 ng mL-1. The recovery values are 96.44-104.39%, with a relative standard deviation 0.15-1.37%. For confirmation of the obtained results, they were statistically compared with a previously published HPLC method utilizing t-and F-tests. © 2021 by the authors.